This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement. Avantages des systèmes d’expression procaryotes ¾Croissance rapide et peu onéreuse (1 génération toutes les 20-30 min pour E. Coli), non pathogènes pour l’homme ¾Système génétique bien connu, nombreuses souches améliorées pour optimiser l’expression de la protéine ¾Expression des gènes facilement contrôlable Systems for Recombinant Protein Expression Lecture Notes Handout • High efficiency method- (electroporation) if low abundant or problems with selection of plasmid • Bacterial strain for DNA purification (DH5a, XL1-Blue or others) which are low in recombinases (RecA-) Popular protein expression systems include E. coli, yeast, baculovirus-infected insect cells, and cultured mammalian cell lines (see Chapter 5).If the requirement is to obtain a protein post-translationally modified via glycosylation (see Chapter 12) or phosphorylation (see Chapter 13), then a eukaryotic expression system must be used. •Wide choice of cloning vectors. E. coli. The Bac-to-Bac™ Baculovirus Expression System The Bac-to-Bac™ Baculovirus Expression System provides a rapid and highly effective method to generate recombinant baculoviruses based on site-specific transposition of an expression cassette into a baculovirus shuttle vector (bacmid) propagated in E. coli. Introduction The E. coli Expression System with Gateway® Technology contains a series of Gateway®-adapted destination vectors designed to facilitate high-level, inducible expression of recombinant proteins in E. coli using the pET system. •Easy to grow with high yields. exonbio provides bacterial expression with the following advantage: One-stop shopping from gene synthesis or gene cloning, expression, and purification; Optimized expression with inducer, temperature, and time course. Depending on •No post-translational modification. •Product can be designed for secretion into the growth media. Overview. Features of Host--prokaryotic systems ex: E coli Advantages Disadvantages •Many references and much experience available e.g. Protein Expression Protocol & Troubleshooting In terms of recombinant expression, E. coli has always been the preferred microbial cell factory as it has multiple, significant benefits over other expression systems including cost, ease-of-use, and scale. A bacterial host is the most widely used expression system due to its simple procedure and low cost. Keywords: recombinant protein expression, Escherichia coli, expression plasmid, inclusion bodies, affinity tags, E. coli expression strains INTRODUCTION There is no doubt that the production of recombinant proteins in microbial systems has revolutionized biochemistry. Choosing an Expression System . •Gene expression easily controlled.

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